Hızlı Islah Teknolojisi Kullanılarak Ekmeklik Buğdayda Rekombinant Kendilenmiş Hat Popülasyonunun Geliştirilmesi

Yazarlar

  • Bedrettin Demir
  • Yaşar Karaduman
  • Nevzat Aydın

DOI:

https://doi.org/10.55205/joctensa.12202269

Anahtar Kelimeler:

Buğday- Hızlı Islah- Çavdar Translokasyonu

Özet

Çavdar translokasyonu buğday ıslahında yaygın olarak kullanılan önemli bir genetik kaynak olmakla birlikte buğdayın ekmeklik kalitesini olumsuz yönde etkilemektedir. Bu araştırmada farklı Yüksek ve Düşük Molekül ağırlıklı glütenin alt ünite (YMA-GA, DMA-GA) kombinasyonları taşıyan aynı zamanda çavdar translokasyonu taşıyıp taşımamasına göre genetik bir açılım gözlenen rekombinant kendilenmiş hat popülasyonu geliştirilmiştir. Rekombinant kendilenmiş hat popülasyonunun geliştirilmesinde hızlı ıslah teknolojisi ve tek tohum soy yöntemi kullanılmıştır. Bir generasyon süresi 50-70 gün arasında değişmiştir. F5:6 generasyonunda 287 adet kendilenmiş hattın elde edildiği araştırmada genetik olarak beklenen 16 farklı YMA-GA ve DMA-GA kombinasyonu gözlenmiştir. Geliştirilen hatların 99 adedi 1BL.1RS çavdar translokasyonu taşımaktadır. Protein kalitesinin belirlenmesi için yapılan Gluten Şişme İndeksi (GSI) analizi sonucunda Glu-B3b alleli taşıyan hatların 3,089 değerine sahip iken, çavdar translokasyonu taşıyan (Glu-B3j alleli) hatların 3,013 değerine sahip olduğu saptanmıştır. Protein oranı ise Glu-B3b alleli taşıyan hatlarda %21,41, Glu-B3j alleli taşıyan hatlarda %20,33 olduğu saptanmıştır. Elde edilen rekombinant kendilenmiş hat popülasyonu çavdar translokasyonu ile YMA-GA ve DMA-GA ünitelerinin ekmeklik buğdayın kalite özelliklerine etkisinin araştırılabileceği materyal olarak kullanılabilir. Araştırma sonuçları hızlı ıslah teknolojisinin buğdayda kısa sürede araştırma materyali geliştirmek için etkin olarak kullanılabileceğini göstermiştir.

Kaynakça

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Yayınlanmış

2022-12-01

Nasıl Atıf Yapılır

Demir , B. ., Karaduman, Y. ., & Aydın, N. (2022). Hızlı Islah Teknolojisi Kullanılarak Ekmeklik Buğdayda Rekombinant Kendilenmiş Hat Popülasyonunun Geliştirilmesi . Cihannüma Teknoloji Fen Ve Mühendislik Bilimleri Akademi Dergisi, 1(2), 36–57. https://doi.org/10.55205/joctensa.12202269

Sayı

Bölüm

Araştırma Makaleleri